The ivory and white garment, designed by Sarah Burton for Alexander McQueen, won over the fashion press and public when Middleton, now the Duchess of Cambridge and a future queen, walked up the aisle of Westminster Abbey in April.
In the run-up to the fairytale wedding, details of the outfit were a closely guarded secret, known only to the handful of people who worked on the dress.
Hundreds of millions of viewers admired it on television and the internet, and now hundreds of thousands will see it for themselves, unprotected on a raised oval stage in the ornate palace ballroom where state banquets are held.
Reflecting the surge in interest in the royals after the marriage and the newlyweds' recent trip to North America, advanced ticket sales for the summer tour of Buckingham Palace have hit 126,000, up 107 percent on the same point last year.
In total a record 643,000 people are expected to take the tour, during which visitors can see 19 state rooms, a display of Faberge jewellery -- and the dress, Middleton's wedding shoes, earrings and a silk replica of her bouquet.
Above the dress is the original veil and Cartier "halo" tiara worn by the bride, featuring around 900 diamonds and lent to her for the occasion by the queen.
Even the wedding cake is on show in a glass cabinet in the state dining room, complete with ornate sugar flowers and a deep cut where the couple plunged in the knife.
The other major attraction for visitors this year is a display of around 100 pieces from the British monarchy's unparalleled collection of works by Russian jeweller Faberge.
The exhibition, which features highlights from a total of 600-700 Faberge creations owned by the royals, ranged from treasures bought by and given to Queen Victoria to a recent bequest to heir to the throne Prince Charles.
The summer opening at Buckingham Palace runs from July 23 to October 3.